Falsely claiming that the acceptance and inclusion of transgender people within the Armed Forces is detrimental to unit cohesion, this order seeks to revive President Trump's trans military ban, which was challenged and blocked numerous times under the first Trump administration. The order states that the policy of the United States Government is to exclude from military service people with gender dysphoria and people who use pronouns that do not reflect their sex assigned at birth. The order directs the Secretary of Defense to promptly issue directives to the Department of Defense revoking inclusive pronoun usage and states that the Armed Forces shall not allow people to use sex-segregated facilities that do not align with their sex assigned at birth.

Journalist Erin Reed reports that a leading transgender military advocacy organization, SPARTA, estimates this "would result in the loss of an $18 billion capital investment, with the Palm Center projecting an additional $1 billion cost to recruit and train replacements. Notably, up to 73% of these service members are senior enlisted personnel with 12-21 years of experience—expertise that cannot be easily replaced by the U.S. government.”